Lab

Consisting of 880 square feet in area, Dr. Siddiqui's Cellular Biochemistry laboratory is located on the 2nd and 5th floors of the Noyes Research Building in Rooms E526 & E208. Other support areas, such as a hot lab, a cold room, and a tissue culture room, are also available to investigators. Cellular Biochemistry Laboratory houses eight Pentium IV computers for equipment operation, graphics processing and data storage.

Dr. Siddiqui's laboratory is equipped to perform many different state-of-the-art techniques. We have tissue culture facility to isolate, culture, grow and maintain primary cells and normal and cancer cells lines.

Our laboratory routinely performs following assays

  • Cell cycle analysis
  • Apoptosis assays
  • Cell proliferation assays
  • Cell migration assays
  • Intra-/extracellular calcium mobilization
  • Protein kinase and phosphatase assays
  • Biochemical characterization of cardiac hypertrophy
  • Protein purification
  • Lipid and fatty acid analysis

Equipment

  • Nuaire biosafety cabinets, incubators
  • Perkin Elmer UV/VIS spectrometer
  • Perkin Elmer Luminescence spectrometer
  • Pharmacia 1-D and 2-D electrophoresis system
  • Powerblock II thermocycler
  • Beckman J-6M centrifuge
  • Eppendorf refrigerated microfuge
  • Olympus CK40 microscope
  • Olympus BX40 microscopes
  • Leica DMI 4000 microscope
  • Leica S8APO microscope
  • Becton Dickinson FACSCalibur Flow Cytometer
  • Becton Dickinson FACStar plus Flow Cytometer
  • Kodak Image Station IS2000MM
  • Optronics Microfire digital camera
  • Shimadzu 2010 series gas chromatography equipment.
  • Shimadzu Prominence LC system